Objective: The study aims to assess the correlation of serum estradiol levels with the number of mature follicles, oocytes retrieved and mature oocytes and pregnancy rates in ART.

Materials and methods: An observational retrospective study was conducted in 2 years (2023-2024) in Ridge IVF centre, New Delhi, India. 200 women of age 23-40 years undergoing IVF/ICSI were included. Correlation of Serum Estradiol on the day of trigger to the number of mature follicles (size ≥ 14mm), the number of oocytes retrieved, the number of mature oocytes, S.AMH, Estradiol per oocyte and Estradiol per mature oocyte on the day of trigger were assessed. The correlation of FORT and FOI with S.AMH levels and S.Estradiol levels were also assessed.

Results: The S.Estradiol levels was positively correlated with mature follicles, oocytes retrieved and mature oocytes. Estradiol-to-oocyte and estradiol-to-mature oocyte ratios both increase with rising estradiol levels. 105 achieved a positive pregnancy, while 95 did not. The mean S.E2 in the positive pregnancy group was 3444.4 ± 2780.35 pg/mL, compared to 3166.4 ± 2571.35 pg/mL in the negative pregnancy group and was not statistically significant.

Conclusion: The present study demonstrates that serum estradiol (E2) on the trigger day reflects not only follicular quantity but also the functional competency of granulosa cells when interpreted alongside established markers of ovarian reserve and ovarian sensitivity. However, Serum E2 alone did not predict clinical pregnancy, highlighting the need for a composite, physiology based approach.

Sakshi Chopra is a renowned Gynaecologist and Infertility specialist in New Delhi, India. She has treated over 3000+ couples with infertility. She has over 12 years’ experience in field of Obstetrics, Gynaecology and Reproductive Medicine. She is currently working as a Senior Consultant at Kailash IVF Hospital, Noida. She has published various articles in national and international journals. She has dedicated her clinical career to advancing evidence based fertility care, with particular interest in individualized ovarian stimulation protocols, recurrent implantation failure, poor ovarian reserve management, PCOS-related infertility, and high-risk reproductive cases. In addition to her clinical work, she actively contributes to academic discussions and professional development initiatives within the field of reproductive medicine. She is passionate about improving reproductive outcomes through innovation, precision medicine, and compassionate care, and she is honoured to share her experience and insights with esteemed colleagues at this international forum.